Share and Export
Share and Export
Ao, Xiang, Bright, Sandra A., Taylor, N.C. and Elmes, Robert B.P. (2017) 2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo. Organic & Biomolecular Chemistry, 15. pp. 6104-6108. ISSN 1477-0520
Preview
RE-nictroreductase-2017.pdf
Download (1MB) | Preview
Abstract
Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.
| Item Type: | Article |
|---|---|
| Additional Information: | Cite as: Org. Biomol. Chem., 2017, 15, 6104 |
| Keywords: | 2-Nitroimidazole; fluorescent probes; nitroreductase; monitoring reductive stress; HeLa cells; |
| Academic Unit: | Faculty of Science and Engineering > Chemistry |
| Item ID: | 11631 |
| Identification Number: | 10.1039/C7OB01406F |
| Depositing User: | Robert Elmes |
| Date Deposited: | 05 Nov 2019 15:25 |
| Journal or Publication Title: | Organic & Biomolecular Chemistry |
| Publisher: | Royal Society of Chemistry |
| Refereed: | Yes |
| Related URLs: | |
| URI: | https://mural.maynoothuniversity.ie/id/eprint/11631 |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
Repository Staff Only (login required)
- Item control page
Downloads
Downloads per month over past year
