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Sedgwick, Adam C., Hayden, Alex, Hill, Barry, Bull, Steven D., Elmes, Robert B.P. and James, Tony D. (2018) A simple umbelliferone based fluorescent probe for the detection of nitroreductase. Frontiers of Chemical Science and Engineering, 12. pp. 311-314. ISSN 2095-0187
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Abstract
A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.
| Item Type: | Article |
|---|---|
| Keywords: | Off-On Probe; Hytixic Tumor; Cancer Therapy; Living Cells; Peroxynitrite; |
| Academic Unit: | Faculty of Science and Engineering > Chemistry |
| Item ID: | 12530 |
| Identification Number: | 10.1007/s11705-017-1697-0 |
| Depositing User: | Robert Elmes |
| Date Deposited: | 06 Mar 2020 16:52 |
| Journal or Publication Title: | Frontiers of Chemical Science and Engineering |
| Publisher: | Springer |
| Refereed: | Yes |
| Related URLs: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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