Objective Many healthcare shift workers do not meet recommended physical activity guidelines. As a pillar of physical activity, this study explored exercise habits, motivators, and barriers encountered by healthcare shift workers. Methods Twelve healthcare shift workers in the United Kingdom were interviewed via semi-structured, one-to-one interviews. Data was analyzed using reflexive thematic analysis. Results Healthcare shift workers experience multiple biopsychosocial barriers to exercise, including inconsistent working schedules, psychophysical fatigue, emotionally challenging shifts, and familial commitments. Exercise habits and motivators were highly individualized. Occupational promotion of exercise, including on-site exercise opportunities, education on exercise prescription, and financially incentivized exercise programs were enablers. Time management and planning were crucial for sustained exercise engagement. Conclusions Organizations requiring shift work should promote a breadth of exercise types, and on-site exercise opportunities, at a low economic burden to promote physical activity engagement within their workforce.