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Yan, Shi, Vanbeselaere, Jorick, Ives, Callum, Stenitzer, David, Nuschy, Lena, Wöls, Florian, Paschinger, Katharina, Fadda, Elisa, Stadlmann, Johannes and Wilson, Iain B.H. (2025) Glycoproteomic and Single-Protein Glycomic Analyses Reveal Zwitterionic N-Glycans on Natural and Recombinant Proteins Derived From Insect Cells. Molecular & Cellular Proteomics, 24 (6). p. 100981. ISSN 1535-9476
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Abstract
Insect cell lines are frequently
used for expression of
recombinant glycoproteins,
including vaccines. Using two
independent approaches, we
show that the endogenous Nglycomes of High Five and Sf9
cells contain species-specific
anionic N-glycan structures with
different combinations of
phosphorylcholine, fucose,
hexuronic acid and/or pentose.
Glycans with phosphorylcholine
and fucose epitopes were also
found on influenza hemagglutinin
and SARS-CoV-2 Spike
expressed in either cell line. Our
data highlight the need for open
search strategies for identifying
glycans present on recombinant
glycoproteins from nonmammalian sources.
| Item Type: | Article |
|---|---|
| Keywords: | N-glycans; baculovirus; fucose; glucuronic acid; insect cells; phosphorylcholine; |
| Academic Unit: | Faculty of Science and Engineering > Chemistry |
| Item ID: | 21251 |
| Identification Number: | 10.1016/j.mcpro.2025.100981 |
| Depositing User: | Elisa Fadda |
| Date Deposited: | 26 Feb 2026 16:27 |
| Journal or Publication Title: | Molecular & Cellular Proteomics |
| Publisher: | Wiley |
| Refereed: | Yes |
| Related URLs: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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