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Singh, Ishwar, Freeman, Colin and Heaney, Frances (2011) Efficient Synthesis of DNA Conjugates by Strain-Promoted Azide–Cyclooctyne Cycloaddition in the Solid Phase. European Journal of Organic Chemistry, 33. pp. 6739-6746. ISSN 1434-193X
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Abstract
Conjugation of ligands to DNA oligonucleotides has been achieved in the solid phase by strain-promoted azide–alkyne cycloaddition (SPAAC). The oligonucleotide, modified with a simple nonfluroinated, monocyclic octyne, efficiently forms conjugates with a range of azide dipoles with varying steric and electronic characteristics. The reaction is clean and easily executed in a copper free environment at room temperature. It provides a variety of triazole-linked nucleic acid conjugates and is potentially useful in biotechnology and cell biology.
| Item Type: | Article |
|---|---|
| Keywords: | Medicinal chemistry; Oligonucleotides; DNA; Solid-phase synthesis; Click reaction; Azides; Alkynes; Cycloaddition; Bioconjugation; |
| Academic Unit: | Faculty of Science and Engineering > Chemistry |
| Item ID: | 7750 |
| Identification Number: | 10.1002/ejoc.201101045 |
| Depositing User: | Dr. Frances Heaney |
| Date Deposited: | 13 Jan 2017 14:23 |
| Journal or Publication Title: | European Journal of Organic Chemistry |
| Publisher: | Wiley |
| Refereed: | Yes |
| Funders: | Science Foundation Ireland (SFI), Irish Research Council for Science Engineering and Technology (IRCSET) |
| Related URLs: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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