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    Proteomic analysis of Bayvarol® resistance mechanisms in the honey bee parasite Varroa destructor


    Surlis, Carla and Carolan, James C. and Coffey, Mary F. and Kavanagh, Kevin (2016) Proteomic analysis of Bayvarol® resistance mechanisms in the honey bee parasite Varroa destructor. Journal of Apicultural Research, 55 (1). pp. 49-64. ISSN 2078-6913

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    Abstract

    The haemophagous mite, Varroa destuctor is one of the most dangerous threats to the Western honey bee, Apis mellifera. Varroa mites parasitize the larval and adult stages of the honey bee and can have devastating effects on the health of the individual bee and colony. In recent years, varroa have shown resistance to the pyrethroid group of insecticides, including Bayvarol® which has flumethrin as the active ingredient. In the work presented here, changes in the expressed proteomes of mites, either sensitive or resistant to Bayvarol® were observed using 2D-SDS-PAGE and shotgun label-free proteomics. A number of detoxification proteins (e.g., glutathione-s-transferase, flavin-containing monooxygenase) were present at higher levels in the resistant mites, as were some proton pumping proteins (e.g., Na+/K+ ATPase alpha and beta subunit, E1–E2 ATPase protein). A decrease in the abundance of 12 cuticle proteins in the resistant mites was observed indicating that alteration to cuticle structure could be a potential resistance mechanism. A number of structural proteins such as myosin and alpha tubulin were expressed at higher levels in the resistant mites, which could indicate a change to the intracellular structure of the cuticle barrier or a change in the cell shape/surface, rather than the addition of extra cuticle proteins. The results presented here indicate higher levels of protein associated with cellular detoxification in Bayvarol®-resistant varroa mites.

    Item Type: Article
    Keywords: Apis mellifera; Bayvarol®; proteomics; pyrethroid; resistance; Varroa destructor;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11098
    Identification Number: https://doi.org/10.1080/00218839.2016.1196015
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 25 Sep 2019 14:15
    Journal or Publication Title: Journal of Apicultural Research
    Publisher: Taylor & Francis
    Refereed: Yes
    Funders: Irish Research Council (IRC), Federation of Irish Beekeepers Associations
    URI:

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