Kelly, Judy and Kavanagh, Kevin
(2010)
Proteomic analysis of proteins released from growth-arrested Candida albicans following exposure to caspofungin.
Medical Mycology, 48 (4).
pp. 598-605.
ISSN 1460-2709
Abstract
The echinocandins (e.g., caspofungin) are a relatively new class of antifungal drugs that function by inhibiting the synthesis of β-1,3-glucan in the cell wall and thus lead to lysis of the cell. In this work the effect of caspofungin on the release of peptides from non-growing cells of the yeast Candida albicans that had been exposed to the drug was monitored. Exposure to 0.19 μg/ml caspofungin resulted in the release of amino acids from cells and of both small and large molecular weight proteins as demonstrated by 1- and 2-dimensional gel electrophoresis. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-ToF) mass spectrometry was employed to identify a number of escaped peptides that were found to have increased in intensity upon exposure to the drug. A number of wall-associated proteins (e.g., phosphoglycerate kinase) and a number of glycolytic enzymes (phosphoglycerate mutase 1, fructose-bisphosphate aldolase) were identified. Importantly, several released proteins (e.g., pyruvate kinase, enolase 1, phosphoglycerate mutase, glyceraldehydes 3-phosphate dehydrogenase, fructose bisphosphate aldolase and alcohol dehydrogenase 1) are highly immunogenic in nature. The results presented here demonstrate that non-growing C. albicans cells are susceptible to the effect of caspofungin and that the caspofungin-mediated release of proteins from such cells could lead to a stronger immune response in vivo. This report illustrates that, in addition to hampering cell wall synthesis, caspofungin may also interfere with the permeability of the fungal cell wall.
| Item Type: |
Article
|
| Keywords: |
antifungal; Candida; caspofungin; proteomics; proteins; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
11109 |
| Identification Number: |
https://doi.org/10.3109/13693780903405782 |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
01 Oct 2019 13:34 |
| Journal or Publication Title: |
Medical Mycology |
| Publisher: |
ISHAM (International Society for Human and Animal Mycology) |
| Refereed: |
Yes |
| Funders: |
Higher Education Authority (HEA) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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