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    Expression of a Barley Peroxidase in Transgenic Apple (Malus domestica L.) Results in Altered Growth, Xylem Formation and Tolerance to Heat Stress


    Vicuna Requesens, Deborah and Malone, Renee P. and Dix, Philip (2014) Expression of a Barley Peroxidase in Transgenic Apple (Malus domestica L.) Results in Altered Growth, Xylem Formation and Tolerance to Heat Stress. Journal of Plant Sciences, 9 (2). pp. 58-66. ISSN 1816-4951

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    Abstract

    Heterologous expression of peroxidase genes has been shown to influence morphology and stress responses of several crop plants but little is known about the effect in woody species. In this study, a barley cell-wall peroxidase gene (prx8), peviously shown to influence growth and stress tolerance in tobacco, was introduced into the genome of apple (Malus domestica cv. Greensleeves) via Agrobacterium-mediated transformation and the presence of the transgene confirmed by PCR and Southern blot analysis. The transgenic plants had up to 4-fold higher levels of peroxidase activity compared to wild type plants and exhibited faster growth and increased xylem production. Leaf discs were incubated at high temperatures (44°C) and electrolyte leakage measurements indicated enhanced tolerance against temperature stress. This effect was confirmed when whole plants were subjected to heat stress after 6 and 12 months growth in soil. These results indicate a link between peroxidase activity levels and resistance to thermal stress, as well as biochemical and physiological changes, in this woody plant.

    Item Type: Article
    Additional Information: The definitive version of this article is available at http://scialert.net/abstract/?doi=jps.2014.58.66
    Keywords: Expression; Barley Peroxidase; Transgenic Apple; Malus domestica L.; Altered Growth; Xylem Formation; Tolerance; Heat Stress;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 5627
    Identification Number: https://doi.org/10.3923/jps.2014.58.66
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 16 Dec 2014 17:08
    Journal or Publication Title: Journal of Plant Sciences
    Publisher: Academic Journals Inc.
    Refereed: Yes
    URI:

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