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    Myricetin Prevents Fibrillogenesis of Hen Egg White Lysozyme


    He, Jianwei and Wang, Yu and Chang, Alan K. and Xu, Linan and Wang, Na and Chong, Xiaoying and Li, Hui and Zhang, Bing and Jones, Gary W. and Song, Youtao (2014) Myricetin Prevents Fibrillogenesis of Hen Egg White Lysozyme. Journal of Agricultural and Food Chemistry, 62. pp. 9442-9449. ISSN 0021-8561

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    Abstract

    Myricetin is a natural flavonol found in many grapes, berries, fruits, vegetables, and herbs as well as other plants. Recent studies have identified potential antiamyloidogenic activity for this compound. In this study, the kinetics of amyloid fibril formation by hen egg white lysozyme (HEWL) and the antifibril-forming activity of myricetin were investigated. We demonstrate that myricetin significantly inhibits the fibrillation of HEWL and the inhibitory effect is dose-dependent. Interestingly, the inhibitory effect toward HEWL fibrillation was stronger than that exerted by the previously characterized fibril-forming inhibitor quercetin, which has high structural similarity with myricetin. Spectrofluorometric and computational studies suggest that the mechanism underlying the inhibitory action of myricetin at a molecular level is to reduce the population of partially unfolded HEWL intermediates. This action is achieved by the tight binding of myricetin to the aggregation-prone region of the β-domain of HEWL and linking to the relatively stable α-domain, thus resulting in the inhibition of amyloid fibril formation.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1021/jf5025449
    Keywords: myricetin; lysozyme; amyloid; molecular dynamics simulation; molecular docking;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 6864
    Identification Number: https://doi.org/10.1021/jf5025449
    Depositing User: Dr. Gary Jones
    Date Deposited: 19 Jan 2016 15:28
    Journal or Publication Title: Journal of Agricultural and Food Chemistry
    Publisher: ACS
    Refereed: Yes
    URI:

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