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    Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress


    Dowling, Paul and O'Driscoll, Lorraine and O'Sullivan, Finbarr and Dowd, Andrew and Henry, Michael and Jeppesen, Per Bendix and Meleady, Paula and Clynes, Martin (2006) Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. Proteomics, 6 (24). pp. 6578-6587. ISSN 1615-9853

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    Abstract

    The glucose-sensitive insulin-secretion (GSIS) phenotype is relatively unstable in long-term culture of beta cells. The purpose of this study was to investigate relative changes in the proteome between glucose-responsive (low passage) and glucose non-responsive (high passage) murine MIN-6 pancreatic beta cells. The 2D-DIGE and subsequent DeCyder analysis detected 3351 protein spots in the pH range of 4–7. Comparing MIN-6(H) to MIN-6(L) and using a threshold of 1.2-fold, the number of proteins with a decrease in expression level was 152 (4.5%), similar was 3140 (93.7%) and increased 59 (1.8%). From the differentially expressed proteins identified in this study, groups of proteins associated with the endoplasmic reticulum (ER) and proteins involved in oxidative stress were found to be significantly decreased in the high-passage (H passage) cells. These proteins included endoplasmic reticulum protein 29 (ERp29); 78-kDa glucoserelated protein, (GRP78); 94-kDa glucose-related protein (GRP94); protein disulphide isomerase; carbonyl reductase 3; peroxidoxin 4 and superoxide dismutase 1. These results suggest that non- GSIS MIN-6 cells do not have the same ability/capacity of glucose-responsive MIN-6 cells to successfully fold, modify or secrete proteins and counteract the problems associated with oxidative stress.

    Item Type: Article
    Keywords: DIGE; Endoplasmic reticulum; Glucose-Sensitive Insulin Secretion; MIN-6 beta cells; Oxidative stress;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7350
    Identification Number: https://doi.org/10.1002/pmic.200600298
    Depositing User: Paul Dowling
    Date Deposited: 16 Aug 2016 15:43
    Journal or Publication Title: Proteomics
    Publisher: Wiley
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    URI:

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