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    Immunogenicity of chloroplast-derived HIV-1 p24 and a p24-Nef fusion protein following subcutaneous and oral administration in mice


    Gonzalez-Rabade, Nuria and McGowan, Edward G. and Zhou, Fei and McCabe, Matthew and Bock, Ralph and Dix, Philip and Gray, John C. and Ma, Julian K-C. (2011) Immunogenicity of chloroplast-derived HIV-1 p24 and a p24-Nef fusion protein following subcutaneous and oral administration in mice. Plant Biotechnology Journal, 9 (6). pp. 629-638. ISSN 1467-7644

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    Abstract

    High-level expression of foreign proteins in chloroplasts of transplastomic plants provides excellent opportunities for the development of oral vaccines against a range of debilitating or fatal diseases. The HIV-1 capsid protein p24 and a fusion of p24 with the negative regulatory protein Nef (p24-Nef) accumulate to ∼4% and ∼40% of the total soluble protein of leaves of transplastomic tobacco (Nicotiana tabacum L.) plants. This study has investigated the immunogenicity in mice of these two HIV-1 proteins, using cholera toxin B subunit as an adjuvant. Subcutaneous immunization with purified chloroplast-derived p24 elicited a strong antigen-specific serum IgG response, comparable to that produced by Escherichia coli-derived p24. Oral administration of a partially purified preparation of chloroplast-derived p24-Nef fusion protein, used as a booster after subcutaneous injection with either p24 or Nef, also elicited strong antigen-specific serum IgG responses. Both IgG1 and IgG2a subtypes, associated with cell-mediated Th1 and humoral Th2 responses, respectively, were found in sera after subcutaneous and oral administration. These results indicate that chloroplast-derived HIV-1 p24-Nef is a promising candidate as a component of a subunit vaccine delivered by oral boosting, after subcutaneous priming by injection of p24 and/or Nef.

    Item Type: Article
    Additional Information: © 2011 The Authors
    Keywords: chloroplast; HIV; oral vaccine; p24; Nef; tobacco;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7366
    Identification Number: https://doi.org/10.1111/j.1467-7652.2011.00609.x
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 23 Aug 2016 15:40
    Journal or Publication Title: Plant Biotechnology Journal
    Publisher: Blackwell Publishing Ltd
    Refereed: Yes
    URI:

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