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    Transformation of potato via Agrobacterium coated microparticle bombardment


    Nguyen, T.T. and Dix, Philip and Nugent, Gregory D. (2010) Transformation of potato via Agrobacterium coated microparticle bombardment. Biologia Plantarum, 54 (1). pp. 141-144. ISSN 006-3134

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    Abstract

    The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3.

    Item Type: Article
    Keywords: potato; Agrobacterium coated microparticle bombardment; Agrobacterium transformation; ipt gene; Solanum tuberosum; transgenic potato;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7371
    Identification Number: https://doi.org/10.1007/s10535-010-0022-2
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 24 Aug 2016 15:39
    Journal or Publication Title: Biologia Plantarum
    Publisher: Springer Verlag
    Refereed: No
    URI:

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