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Nguyen, T.T., Dix, Philip and Nugent, Gregory D. (2010) Transformation of potato via Agrobacterium coated microparticle bombardment. Biologia Plantarum, 54 (1). pp. 141-144. ISSN 006-3134
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Abstract
The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated
biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved
through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in
Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine
shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively
increasing concentrations of hygromycin from 5 to 20 mg dm-3.
| Item Type: | Article |
|---|---|
| Keywords: | potato; Agrobacterium coated microparticle bombardment; Agrobacterium transformation; ipt gene; Solanum tuberosum; transgenic potato; |
| Academic Unit: | Faculty of Science and Engineering > Biology |
| Item ID: | 7371 |
| Identification Number: | 10.1007/s10535-010-0022-2 |
| Depositing User: | Prof. Philip J. Dix |
| Date Deposited: | 24 Aug 2016 15:39 |
| Journal or Publication Title: | Biologia Plantarum |
| Publisher: | Springer Verlag |
| Refereed: | No |
| Related URLs: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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