Elramli, Nadia
(2017)
Role of Striatin Complexes in the Control
of Development and Secondary
Metabolism of the Filamentous Fungus
Aspergillus nidulans.
PhD thesis, National University of Ireland Maynooth.
Abstract
Striatin is a highly conserved eukaryotic protein first discovered in neuron
cells of mammals. It is involved in many molecular processes, including
cellular signaling, cell division and development. It has been shown in
several fungi that striating complexes are important for development.
Fungal development and secondary metabolism (SM) requires regulatory
complexes which are under control of the environmental signals such as
light, CO2 and pH . Aspergillus nidulans is a model for eukaryotic systems
as well as fungal development and SM. It produces more than forty
identified compounds with potent biological activities. Function of the
striatin gene, strA was previously shown to influence development in A.
nidulans. However, detailed molecular function of StrA in fungal
development as well as SM production is still unknown. This study focuses
on the interactome of striatin protein by using tandem affinity purification
(TAP) and green fluorescent protein (GFP) tags in A. nidulans. Five
proteins interacting or associated with StrA protein were identified TAP and
GFP-TRAP by using mass spectrometry (MS). They were named as StrA
interacting proteins (Sips), SipA, SipB, SipC, SipD and SipE. The single
and double deletion combinations of all sipA, sipB, sipC, sipD and sipE
genes led to serious developmental as well as SM defects in A. nidulans.
Localization and pull-down experiments suggest that StrA is required for
the full assembly of the functional STRIPAK complex at the nuclear
envelope. Furthermore, assembly dynamics of the STRIPAK complex was
also determined. StrA interacts with SipA constantly during all
developmental stages. However, SipB-SipD and SipC-SipE are found as
heterodimers, which later assemble to StrA-SipA core heterodimer, which
constitute the hexameric STRIPAK complex. This study determines the
presence of STRIPAK complex by biochemical, genetic and cell biological
methods, which is required for light responses, fungal fruit body formation,
asexual conidiation and secondary metabolite SM production.
| Item Type: |
Thesis
(PhD)
|
| Keywords: |
Striatin Complexes; Control
of Development; Secondary
Metabolism; Filamentous Fungus;
Aspergillus nidulans; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
10408 |
| Depositing User: |
IR eTheses
|
| Date Deposited: |
09 Jan 2019 09:16 |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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