Moloney, Nicola M. and Owens, Rebecca A. and Meleady, Paula and Henry, Michael and Dolan, Stephen K. and Mulvihill, Eoin and Clynes, Martin and Doyle, Sean (2016) The iron-responsive microsomal proteome of Aspergillus fumigatus. Journal of Proteomics, 136. pp. 99-111. ISSN 1874-3919

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Abstract

Aspergillus fumigatus is an opportunistic fungal pathogen. Siderophore biosynthesis and iron acquisition are essential for virulence. Yet, limited data exist with respect to the adaptive nature of the fungal microsomal proteome under iron-limiting growth conditions, as encountered during host infection. Here, we demonstrate that under siderophore biosynthetic conditions — significantly elevated fusarinine C (FSC) and triacetylfusarinine C (TAFC) production (p < 0.0001), extensive microsomal proteome remodelling occurs. Specifically, a four-fold enrichment of transmembrane-containing proteins was observed with respect to whole cell lysates following ultracentrifugation-based microsomal extraction. Comparative label-free proteomic analysis of microsomal extracts, isolated following iron-replete and -deplete growth, identified 710 unique proteins. Scatterplot analysis (MaxQuant) demonstrated high correlation amongst biological replicates from each growth condition (Pearson correlation > 0.96 within groups; biological replicates (n = 4)). Quantitative and qualitative comparison revealed 231 proteins with a significant change in abundance between the iron-replete and iron-deplete conditions (p < 0.05, fold change ≥ 2), with 96 proteins showing increased abundance and 135 with decreased abundance following iron limitation, including predicted siderophore transporters. Fluorescently labelled FSC was only sequestered following A. fumigatus growth under iron-limiting conditions. Interestingly, human sera exhibited significantly increased reactivity (p < 0.0001) against microsomal protein extracts obtained following iron-deplete growth.

Item Type:	Article
Keywords:	Iron; Aspergillus fumigatus; Fungal proteomics; Siderophore; Membrane proteins;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	10910
Identification Number:	https://doi.org/10.1016/j.jprot.2015.12.025
Depositing User:	Rebecca Owens
Date Deposited:	02 Jul 2019 14:00
Journal or Publication Title:	Journal of Proteomics
Publisher:	Elsevier
Refereed:	Yes
URI:	https://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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