Kenna, Tony and O'Brien, Margaret and Hogan, Andrew E. and Exley, Mark A. and Porcelli, Steven A. and Hegarty, John E. and O'Farrelly, Cliona and Doherty, Derek G. (2007) CD1 expression and CD1-restricted T cell activity in normal and tumour-bearing human liver. Cancer Immunology, Immunotherapy, 56 (4). pp. 563-572. ISSN 1432-0851

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Abstract

CD1d-restricted natural killer T (NKT) cells expressing invariant Vα14Jα18 T cell receptor α-chains are abundant in murine liver and are implicated in the control of malignancy, infection and autoimmunity. Invariant NKT cells have potent anti-metastatic effects in mice and phase I clinical trials involving their homologues in humans are ongoing. However, invariant NKT cells are less abundant in human liver (∼0.5% of hepatic T cells) than in murine liver (up to 50%) and it is not known if other hepatic T cells are CD1-restricted. We have examined expression of CD1a, CD1b, CD1c and CD1d mRNA and protein in human liver and evaluated the reactivity of mononuclear cells (MNC) from histologically normal and tumour-bearing human liver specimens against these CD1 isoforms. Messenger RNA for all CD1 isotypes was detectable in all liver samples. CD1c and CD1d were expressed at the protein level by hepatic MNC. CD1d, only, was detectable at the cell surface, but CD1c and CD1d were found at an intracellular location in significant numbers of liver MNC. CD1b was not expressed by MNC from healthy livers but was detectable within MNC in all tumour samples tested. Hepatic T cells exhibited reactivity against C1R cells expressing transfected CD1c and CD1d, but neither CD1a nor CD1b. These cells secreted interferon-γ (IFN-γ) but not interleukin-4 (IL-4) upon stimulation. In contrast, similar numbers of peripheral T cells released 13- and 16-fold less IFN-γ in response to CD1c and CD1d, respectively. CD1c and CD1d expression and T cell reactivity were not altered in tumour-bearing liver specimens compared to histologically normal livers. These data suggest that, in addition to invariant CD1d-restricted NKT cells, autoreactive T cells that recognise CD1c and CD1d and release inflammatory cytokines are abundant in human liver.

Item Type:	Article
Additional Information:	Cite as: Kenna, T., O’Brien, M., Hogan, A.E. et al. Cancer Immunol Immunother (2007) 56: 563. https://doi.org/10.1007/s00262-006-0215-x
Keywords:	Human; Liver; T cells; Natural killer T cells; Tumour immunity;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	10992
Identification Number:	https://doi.org/10.1007/s00262-006-0215-x
Depositing User:	Andrew Hogan
Date Deposited:	27 Aug 2019 13:58
Journal or Publication Title:	Cancer Immunology, Immunotherapy
Publisher:	Springer Verlag
Refereed:	Yes
Funders:	Irish Health Research Board, Science Foundation Ireland, National Institute of Health
URI:	https://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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