MURAL - Maynooth University Research Archive Library

    Activation of human invariant natural killer T cells with a thioglycoside analogue of α-galactosylceramide

    Hogan, Andrew E. and O'Reilly, Vincent P. and Dunne, Margaret R. and Dere, Ravindra T. and Zeng, Shijuan G. and O'Brien, Cashel and Amu, Sylvie and Fallon, Padraic G. and Exley, Mark A. and O'Farrelly, Cliona and Zhu, Xiangming and Doherty, Derek G. (2011) Activation of human invariant natural killer T cells with a thioglycoside analogue of α-galactosylceramide. Clinical Immunology, 140 (2). pp. 196-207. ISSN 1521-6616

    Download (1MB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    Activation of CD1d-restricted invariant NKT (iNKT) cells with the glycolipid α-galactosylceramide (α-GalCer) confers protection against disease in murine models, however, clinical trials in humans have had limited impact. We synthesized a novel thioglycoside analogue of α-GalCer, denoted α-S-GalCer, and tested its efficacy for stimulating human iNKT cells in vitro. α-S-GalCer stimulated cytokine release by iNKT cells in a CD1d-dependent manner and primed CD1d+ target cells for lysis. α-S-GalCer-stimulated iNKT cells induced maturation of monocyte-derived dendritic cells into antigen-presenting cells that released IL-12 and small amounts of IL-10. The nature and potency of α-S-GalCer and α-GalCer in human iNKT cell activation were similar. However, in contrast to α-GalCer, α-S-GalCer did not activate murine iNKT cells in vivo. Because of its enhanced stability in biological systems, α-S-GalCer may be superior to α-GalCer as a parent compound for developing adjuvant therapies for humans.

    Item Type: Article
    Keywords: NKT cells; Dendritic cells; Human; Cytokines; Glycolipids;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 10993
    Identification Number:
    Depositing User: Andrew Hogan
    Date Deposited: 27 Aug 2019 14:57
    Journal or Publication Title: Clinical Immunology
    Publisher: Elsevier
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Health Research Board (HRB), Irish Research Council for Science, Engineering and Technology (IRCSET)
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads