Valsecchi, Isabel and Bayram, Ozlem Sarikaya and Wong Sak Hoi, Joanne and Muszkieta, Laetitia and Gibbons, John and Prevost, Marie‐Christine and Mallet, Adeline and Krijnse‐Locker, Jacomina and Ibrahim‐Granet, Oumaima and Mouyna, Isabelle and Carr, Paul D. and Bromley, Michael and Aimanianda, Vishukumar and Yu, Jae‐Hyuk and Rokas, Antonis and Braus, Gerhard H. and Saveanu, Cosmin and Bayram, Ozgur and Latgé, Jean Paul (2017) MybA, a transcription factor involved in conidiation and conidial viability of the human pathogen Aspergillus fumigatus. Molecular Microbiology, 105 (6). pp. 880-900. ISSN 1365-2958

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Abstract

Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of propagation, survival and infection of this human pathogen. In this study, we present the molecular characterization of a novel regulator of conidiogenesis and conidial survival called MybA because the predicted protein contains a Myb DNA binding motif. Cellular localization of the MybA::Gfp fusion and immunoprecipitation of the MybA::Gfp or MybA::3xHa protein showed that MybA is localized to the nucleus. RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions upstream of wetA, vosA and velB, the key regulators involved in conidial maturation. The deletion of mybA resulted in a very significant reduction in the number and viability of conidia. As a consequence, the ΔmybA strain has a reduced virulence in an experimental murine model of aspergillosis. RNA‐sequencing and biochemical studies of the ΔmybA strain suggested that MybA protein controls the expression of enzymes involved in trehalose biosynthesis as well as other cell wall and membrane‐associated proteins and ROS scavenging enzymes. In summary, MybA protein is a new key regulator of conidiogenesis and conidial maturation and survival, and plays a crucial role in propagation and virulence of A. fumigatus.

Item Type:	Article
Keywords:	MybA; transcription factor; conidiation; conidial viability; Aspergillus fumigatus;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11003
Identification Number:	https://doi.org/10.1111/mmi.13744
Depositing User:	Ozgur Bayram
Date Deposited:	02 Sep 2019 15:21
Journal or Publication Title:	Molecular Microbiology
Publisher:	John Wiley & Sons Ltd
Refereed:	Yes
URI:	https://onlinelibrary.wiley.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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