Graciet, Emmanuelle and Ó'Maoiléidigh, Diarmuid S. and Wellmer, Frank (2014) Next-Generation Sequencing Applied to Flower Development (ChIP-Seq). In: Flower Development. Methods in Molecular Biology (Methods and Protocols) (1110). Humana Press, pp. 413-429. ISBN 9781461494089

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Abstract

Over the past 20 years, classic genetic approaches have shown that the developmental program underlying flower formation involves a large number of transcriptional regulators. However, the target genes of these transcription factors, as well as the gene regulatory networks they control, remain largely unknown. Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-Seq), which allows the identification of transcription factor binding sites on a genome-wide scale, has been successfully applied to a number of transcription factors in Arabidopsis. The ChIP-Seq procedure involves chemical cross-linking of proteins to DNA, followed by chromatin fragmentation and immunoprecipitation of specific protein–DNA complexes. The regions of the genome bound by a specific transcription factor can then be identified after next-generation sequencing.

Item Type:	Book Section
Additional Information:	This is the preprint version of the published article, which is available at: Graciet E., Ó’Maoiléidigh D.S., Wellmer F. (2014) Next-Generation Sequencing Applied to Flower Development: ChIP-Seq. In: Riechmann J., Wellmer F. (eds) Flower Development. Methods in Molecular Biology (Methods and Protocols), vol 1110. Humana Press, New York, NY
Keywords:	Arabidopsis; flower development; ChIP-Seq; transcription factors;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11024
Identification Number:	https://doi.org/10.1007/978-1-4614-9408-9_24
Depositing User:	Emmanuelle Graciet
Date Deposited:	05 Sep 2019 14:52
Journal or Publication Title:	Flower Development
Publisher:	Humana Press
Refereed:	Yes
URI:	https://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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