Goslin, Kevin and Zheng, Beibei and Serrano-Mislata, Antonio and Rae, Liina and Ryan, Patrick T. and Kwasniewska, Kamila and Thomson, Bennett and Ó'Maoiléidigh, Diarmuid S. and Madueno, Francisco and Wellmer, Frank and Graciet, Emmanuelle (2017) Transcription Factor Interplay between LEAFY and APETALA1/CAULIFLOWER during Floral Initiation. Plant Physiology, 174 (2). pp. 1097-1109. ISSN 1532-2548

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Abstract

The transcription factors LEAFY (LFY) and APETALA1 (AP1), together with the AP1 paralog CAULIFLOWER (CAL), control the onset of flower development in a partially redundant manner. This redundancy is thought to be mediated, at least in part, through the regulation of a shared set of target genes. However, whether these genes are independently or cooperatively regulated by LFY and AP1/CAL is currently unknown. To better understand the regulatory relationship between LFY and AP1/ CAL and to obtain deeper insights into the control of floral initiation, we monitored the activity of LFY in the absence of AP1/ CAL function. We found that the regulation of several known LFY target genes is unaffected by AP1/CAL perturbation, while others appear to require AP1/CAL activity. Furthermore, we obtained evidence that LFY and AP1/CAL control the expression of some genes in an antagonistic manner. Notably, these include key regulators of floral initiation such as TERMINAL FLOWER1 (TFL1), which had been previously reported to be directly repressed by both LFY and AP1. We show here that TFL1 expression is suppressed by AP1 but promoted by LFY. We further demonstrate that LFY has an inhibitory effect on flower formation in the absence of AP1/CAL activity. We propose that LFY and AP1/CAL act as part of an incoherent feed-forward loop, a network motif where two interconnected pathways or transcription factors act in opposite directions on a target gene, to control the establishment of a stable developmental program for the formation of flowers.

Item Type:	Article
Additional Information:	Cite as: Transcription Factor Interplay between LEAFY and APETALA1/CAULIFLOWER during Floral Initiation. Kevin Goslin, Beibei Zheng, Antonio Serrano-Mislata, Liina Rae, Patrick T. Ryan, Kamila Kwaśniewska, Bennett Thomson, Diarmuid S. Ó’Maoiléidigh, Francisco Madueño, Frank Wellmer, Emmanuelle Graciet. Plant Physiology Jun 2017, 174 (2) 1097-1109; DOI: 10.1104/pp.17.00098
Keywords:	Transcription Factor Interplay; LEAFY and APETALA1; CAULIFLOWER; Floral Initiation;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11027
Identification Number:	https://doi.org/10.1104/pp.17.00098
Depositing User:	Emmanuelle Graciet
Date Deposited:	06 Sep 2019 15:54
Journal or Publication Title:	Plant Physiology
Publisher:	American Society of Plant Biologists
Refereed:	Yes
Funders:	Science Foundation Ireland (SFI), MINECO, FEDER, Irish Research Council (IRC)
URI:	http://www.plantphysiol.org
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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