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    Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco

    Dix, Philip and Burbridge, Emma and Diamond, M. and McCabe, P.F. (2006) Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco. Plant Science, 171. pp. 138-146.

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    Tobacco suspension cultures were subjected to a range of heat stresses and used to compare morphological aspects of programmed cell death (PCD) and necrosis. Cells undergoing PCD were found to display characteristic death morphology, caused by cytoplasmic retraction of the protoplast, and to have cleaved DNA. We evaluated if the morphological characteristics of PCD could be used to monitor changes in cell death induction thresholds in transgenic cell cultures with high levels of peroxidase activity. Again, using a heat shock assay, we show that tobacco cell cultures with elevated levels of peroxidase have higher cell death induction threshold levels than wild type tobacco cell cultures. Thus, assessing PCD associated morphological changes can report on the effect of altering peroxidase genes on cell death activation in tobacco. This study demonstrates that PCD morphology could routinely be used to monitor the effects of introduced genes on programmed cell death induction thresholds in plants.

    Item Type: Article
    Keywords: Programmed cell death; Heat-shock; Peroxidase
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 1109
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 18 Dec 2008 16:07
    Journal or Publication Title: Plant Science
    Publisher: Elsevier
    Refereed: Yes
      Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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