Sheehan, Gerard and Kavanagh, Kevin (2018) Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans. Virulence, 9 (1). pp. 163-172. ISSN 2150-5608

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Abstract

Galleria mellonella larvae were administered an inoculum of Candida albicans and the response to infection over 24 hours was monitored. The yeast cell density in infected larvae declined initially but replication commenced six hours post-infection. The hemocyte density decreased from 5.2 × 106/ml to 2.5 × 106/ml at 2 hours but increased to 4.2 × 106 at 6 hours and decreased subsequently. Administration of β – glucan to larvae also caused a fluctuation in hemocyte density (5.1 ± 0.22 × 106/ml (0 hour) to 6.25 ± 0.25 × 106/ml (6 hour) (p < 0.05) to 5 ± 2.7 × 106 (24 hour)) and the population showed an increase in the density of small, granular cells at 24 hours (p < 0.05). Hemocytes from larvae inoculated with β – glucan for 6 or 24 hours showed faster killing of C. albicans cells (53 ± 4.1% (p < 0.01), 64 ± 3.7%, (p < 0.01), respectively) than hemocytes from control larvae (24 ± 11%) at 60 min. Proteomic analysis indicated increased abundance of immune related proteins cecropin-A (5 fold) and prophenoloxidase-activating proteinase-1 (5 fold) 6 hours post infection but by 24 hours there was elevated abundance of muscle (tropomyosin 2 (141 fold), calponin (66 fold), troponin I (62 fold)) and proteins indicative of cellular stress (glutathione-S-transferase-like protein (114 fold)), fungal dissemination (muscle protein 20-like protein (174 fold)) and tissue breakdown (mitochondrial cytochrome c (10 fold)). Proteins decreased in abundance at 24 hour included β – 1,3 – glucan recognition protein precursor (29 fold) and prophenoloxidase subunit 2 (25 fold).

Item Type:	Article
Additional Information:	Cite as: Gerard Sheehan & Kevin Kavanagh (2018) Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans, Virulence, 9:1, 163-172, DOI: 10.1080/21505594.2017.1370174 . © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords:	candida; galleria; immunity; infection; in vivo screening; Mini-model;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11099
Identification Number:	https://doi.org/10.1080/21505594.2017.1370174
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	25 Sep 2019 15:01
Journal or Publication Title:	Virulence
Publisher:	Taylor & Francis Open
Refereed:	Yes
URI:	https://www.tandfonline.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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