Maurer, Elisabeth and Browne, Niall and Surlis, Carla and Jukic, Emina and Moser, Patrizia and Kavanagh, Kevin and Lass-Flörl, Cornelia and Binder, Ulrike (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance. Virulence, 6 (6). pp. 591-598. ISSN 2150-5608

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Abstract

The aim of this study was to investigate if the alternative in vivo model Galleria mellonella can be used (i) to determine differences in pathogenicity of amphotericin B (AMB) resistant and susceptible A. terreus isolates, (ii) to evaluate AMB efficacy in vivo (iii) and to correlate outcome to in vitro susceptibility data. Larvae were infected with 2 A. terreus AMB resistant (ATR) and 3 AMB susceptible (ATS) isolates and survival rates were correlated to physiological attributes and killing ability of larval haemocytes. Additionally, infected larvae were treated with different concentrations of L-AMB. Haemocyte density were ascertained to evaluate the influence of L-AMB on the larval immune cells. Larvae were sensitive to A. terreus infection in an inoculum-size and temperature dependent manner. In vitro susceptibility to L-AMB correlated with in vivo outcome of antifungal treatment, defining an AMB susceptible strain cluster of A. terreus. Susceptibility to L-AMB increased virulence potential in the larval model, but this increase was also in accordance with faster growth and less damage caused by larval haemocytes. L-AMB treatment primed the larval immune response by increasing haemocyte density. G. mellonella provides a convenient model for the in vivo screening of A. terreus virulence and treatment options, contributing to the generation of a hypothesis that can be further tested in refined experiments in mammalian models.

Item Type:	Article
Additional Information:	Cite as: Elisabeth Maurer, Niall Browne, Carla Surlis, Emina Jukic, Patrizia Moser, Kevin Kavanagh, Cornelia Lass-Flörl & Ulrike Binder (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance, Virulence, 6:6, 591-598, DOI: 10.1080/21505594.2015.1045183
Keywords:	amphotericin B resistant and susceptible A. terreus strain cluster; Galleria mellonella; invertebrate in vivo model;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11101
Identification Number:	https://doi.org/10.1080/21505594.2015.1045183
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	25 Sep 2019 16:03
Journal or Publication Title:	Virulence
Publisher:	Taylor & Francis Open
Refereed:	Yes
URI:	https://www.tandfonline.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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