MURAL - Maynooth University Research Archive Library

    Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance

    Maurer, Elisabeth and Browne, Niall and Surlis, Carla and Jukic, Emina and Moser, Patrizia and Kavanagh, Kevin and Lass-Flörl, Cornelia and Binder, Ulrike (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance. Virulence, 6 (6). pp. 591-598. ISSN 2150-5608

    Download (1MB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    The aim of this study was to investigate if the alternative in vivo model Galleria mellonella can be used (i) to determine differences in pathogenicity of amphotericin B (AMB) resistant and susceptible A. terreus isolates, (ii) to evaluate AMB efficacy in vivo (iii) and to correlate outcome to in vitro susceptibility data. Larvae were infected with 2 A. terreus AMB resistant (ATR) and 3 AMB susceptible (ATS) isolates and survival rates were correlated to physiological attributes and killing ability of larval haemocytes. Additionally, infected larvae were treated with different concentrations of L-AMB. Haemocyte density were ascertained to evaluate the influence of L-AMB on the larval immune cells. Larvae were sensitive to A. terreus infection in an inoculum-size and temperature dependent manner. In vitro susceptibility to L-AMB correlated with in vivo outcome of antifungal treatment, defining an AMB susceptible strain cluster of A. terreus. Susceptibility to L-AMB increased virulence potential in the larval model, but this increase was also in accordance with faster growth and less damage caused by larval haemocytes. L-AMB treatment primed the larval immune response by increasing haemocyte density. G. mellonella provides a convenient model for the in vivo screening of A. terreus virulence and treatment options, contributing to the generation of a hypothesis that can be further tested in refined experiments in mammalian models.

    Item Type: Article
    Additional Information: Cite as: Elisabeth Maurer, Niall Browne, Carla Surlis, Emina Jukic, Patrizia Moser, Kevin Kavanagh, Cornelia Lass-Flörl & Ulrike Binder (2015) Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance, Virulence, 6:6, 591-598, DOI: 10.1080/21505594.2015.1045183
    Keywords: amphotericin B resistant and susceptible A. terreus strain cluster; Galleria mellonella; invertebrate in vivo model;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11101
    Identification Number:
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 25 Sep 2019 16:03
    Journal or Publication Title: Virulence
    Publisher: Taylor & Francis Open
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads