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    Dose-dependent cellular and humoral responses in Galleria mellonella larvae following β-glucan inoculation

    Mowlds, Peter and Coates, Christopher and Renwick, Julie and Kavanagh, Kevin (2010) Dose-dependent cellular and humoral responses in Galleria mellonella larvae following β-glucan inoculation. Microbes and Infection, 12 (2). pp. 146-153. ISSN 1286-4579

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    Galleria mellonella larvae were inoculated with different doses of β-glucan by injection into the haemocoel. Those larvae that had received high doses of β-glucan (15, 30 or 60 μg/larva) demonstrated increased survival following infection with the yeast Candida albicans. High concentrations of glucan induced an increase in haemocyte density and a reduction in yeast proliferation within the haemocoel. Proteomic analysis of glucan-treated larvae revealed increased expression of a variety of peptides some of which may possess antimicrobial properties. Analysis of expression profiles revealed that low doses of β-glucan (3.75 μg/larva) triggered the increased expression of certain peptides (e.g. hemolin) while high dose inoculation was required before the increased expression of others (e.g. archaemetzincin) was evident. These results indicate that low doses of β-glucan induce a limited immune response while high doses induce an immune response that has the potential to curtail the threat within the haemocoel but also withstand a subsequent infection. Immune priming gives insects the ability to withstand a potentially lethal infection if exposed to a low level of the pathogen 24–48 h previously. Immune priming has resource implications and this work indicates that a graded immune response is initiated depending upon the amount of the immune priming agent encountered.

    Item Type: Article
    Keywords: Antimicrobial peptides; Galleria larvae; Haemocytes; Insect; Immune response; Proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 11115
    Identification Number:
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 01 Oct 2019 14:30
    Journal or Publication Title: Microbes and Infection
    Publisher: Elsevier
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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