Cotter, Gary and Wheedle, Roisin and Kavanagh, Kevin (1998) Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells. Mycopathologia, 141 (3). pp. 137-142. ISSN 1573-0832

Preview

Download (321kB) | Preview

Abstract

The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM proteins reduced the adherence of C. albicans ATCC 44990 to HEp-2 cells, the greatest reductions being evident in assays which incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans – HEp-2 adherence was in assays which incorporated 0.2M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine reduced the adherence of C. albicans to HEp-2 cells by approximately 70% (p < 0.05).

Item Type:	Article
Additional Information:	Cite as: Cotter, G., Weedle, R. & Kavanagh, K. Mycopathologia (1998) 141: 137. https://doi.org/10.1023/A:1006940203962
Keywords:	Adherence; Candida; Extracellular matrix; HEp-2;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11120
Identification Number:	https://doi.org/10.1023/A:1006940203962
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	03 Oct 2019 14:26
Journal or Publication Title:	Mycopathologia
Publisher:	Kluwer Academic Publishers
Refereed:	Yes
URI:	https://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads