Cotter, Gary and Verhaegen, S. and Clynes, M. and Kavanagh, Kevin (1997) Membrane changes associated with the early stages of apoptosis in HEp-2 cells decrease susceptibility to adherence by Candida albicans. Journal of Medical and Veterinary Mycology, 35 (3). pp. 219-223. ISSN 0268-1218

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Abstract

The aim of the present work was to establish whether apoptotic HEp-2 cells demonstrated an altered susceptibility to adherence by the pathogenic yeast Candida albicans. Cultures of HEp-2 cells were treated with concentrations of three chemotherapeutic agents sufficient to induce cell death by apoptosis and this was confirmed by microscopic examination and by the loss of membrane asymmetry (as indicated by phosphatidylserine externalization) and the fragmentation of nuclear DNA into distinct subunits. Cells in the early stages of apoptosis demonstrated a reduced susceptibility to adherence by a number of strains of C. albicans. A correlation between the decrease in yeast adherence and the loss of membrane asymmetry, associated with the early stages of apoptosis, was established.

Item Type:	Article
Additional Information:	Cite as: G. Cotter, S. Verhaegen, M. Clynes, K. Kavanagh, Membrane changes associated with the early stages of apoptosis in HEp-2 cells decrease susceptibility to adherence by Candida albicans, Journal of Medical and Veterinary Mycology, Volume 35, Issue 3, May 1997, Pages 219–224, https://doi.org/10.1080/02681219780001171
Keywords:	adherence; apoptosis; Candida; HEp-2;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	11121
Identification Number:	https://doi.org/10.1080/02681219780001171
Depositing User:	Dr. Kevin Kavanagh
Date Deposited:	03 Oct 2019 14:46
Journal or Publication Title:	Journal of Medical and Veterinary Mycology
Publisher:	ISHAM (International Society for Human and Animal Mycology)
Refereed:	Yes
URI:	https://academic.oup.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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