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    Development of a microelectrochemical biosensor for the real-time detection of choline

    Baker, Keeley L. and Bolger, Fiachra B. and Lowry, John P. (2017) Development of a microelectrochemical biosensor for the real-time detection of choline. Sensors and Actuators B: Chemical, 243. pp. 412-420. ISSN 0925-4005

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    Here we describe the development of a first generation biosensor for the detection of brain extracellular choline, investigating important considerations for in-vivo monitoring such as sensor sensitivity, O2 interference and selectivity. Extensive optimisation of choline biosensor designs resulted in a biosensor with excellent sensitivity towards choline (0.54 ± 0.03 nA/μM). Oxygen interference studies demonstrate a 1% reduction in current at 50 μM O2 when compared to atmospheric O2 levels (200 μM), indicating that the sensor can be used for reliable choline monitoring, free from changes in current associated with physiological O2 fluctuations. A negligible sensitivity of 0.0021 ± 0.0002 nA/μM n = 8 was achieved utilising poly-phenylenediamine (PPD) as a permselective membrane for interference rejection of ascorbic acid (AA), the most physiologically important endogenous electroactive species present in the brain. The optimised biosensor when implanted into the striatum of a freely moving rat successfully detected local perfusions of choline demonstrating the sensors ability to detect choline in-vivo.

    Item Type: Article
    Keywords: Acetylcholine; Choline; Biosensor; Oxygen interference; Selectivity; Sensitivity;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 11613
    Identification Number:
    Depositing User: John Lowry
    Date Deposited: 05 Nov 2019 17:57
    Journal or Publication Title: Sensors and Actuators B: Chemical
    Publisher: Elsevier
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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