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    Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy


    Murphy, Sandra and Dowling, Paul and Zweyer, Margit and Henry, Michael and Meleady, Paula and Mundegar, Rustam R. and Swandulla, Dieter and Ohlendieck, Kay (2017) Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. International Journal of Molecular Medicine, 39 (6). pp. 1357-1370. ISSN 1791-244X

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    Abstract

    X-linked muscular dystrophy is caused by primary abnormalities in the Dmd gene and is characterized by the almost complete loss of the membrane cytoskeletal protein dystrophin, which triggers sarcolemmal instability, abnormal calcium homeostasis, increased proteolysis and impaired excitation‑contraction coupling. In addition to progressive necrosis, crucial secondary pathologies are represented by myofibrosis and the invasion of immune cells in damaged muscle fibres. In order to determine whether these substantial changes within the skeletal musculature are reflected by an altered rate of protein release into the circulatory system or other plasma fluctuations, we used label‑free mass spectrometry to characterize serum from the mdx‑4cv model of Duchenne muscular dystrophy. Comparative proteomics revealed a large number of increased vs. decreased protein species in mdx‑4cv serum. A serum component with greatly elevated levels was identified as the inflammation‑inducible plasma marker haptoglobin. This acute phase response protein is usually secreted in relation to tissue damage and sterile inflammation. Both immunoblot analyses and enzyme‑linked immunosorbent assays confirmed the increased concentration of haptoglobin in crude mdx‑4cv serum. This suggests that haptoglobin, in conjunction with other altered serum proteins, represents a novel diagnostic, prognostic and/or therapy‑monitoring biomarker candidate to evaluate the inflammatory response in the mdx‑4cv animal model of dystrophinopathy.

    Item Type: Article
    Additional Information: © Murphy et al. This is an open access article distributed under the terms of Creative Commons Attribution License https://creativecommons.org/licenses/by-nc-nd/4.0/ . ite as: Murphy, S., Dowling, P., Zweyer, M., Henry, M., Meleady, P., Mundegar, R. R., Swandulla, D., Ohlendieck, K."Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy". International Journal of Molecular Medicine 39, no. 6 (2017): 1357-1370. https://doi.org/10.3892/ijmm.2017.2952
    Keywords: acute phase plasma protein; biomarker; dystrophin; dystrophinopathy; haptoglobin; sterile inflammation;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 11616
    Identification Number: https://doi.org/10.3892/ijmm.2017.2952
    Depositing User: Paul Dowling
    Date Deposited: 04 Nov 2019 16:38
    Journal or Publication Title: International Journal of Molecular Medicine
    Publisher: Spandidos Publications
    Refereed: Yes
    URI:

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