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    Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications


    Gupta, Saket and Maratha, Ashwini and Siednienko, Jakub and Natarajan, Anandan and Gajanayake, Thusitha and Hoashi, Shu and Miggin, Sinead (2017) Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications. Scientific Reports, 7. p. 7633. ISSN 2045-2322

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    Abstract

    The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups.

    Item Type: Article
    Keywords: Diabetes mellitus (non-insulin dependent); Diabetes mellitus; Inflammation;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 11669
    Identification Number: https://doi.org/10.1038/s41598-017-07230-8
    Depositing User: Sinead Miggin
    Date Deposited: 12 Nov 2019 12:34
    Journal or Publication Title: Scientific Reports
    Publisher: Nature Publishing Group
    Refereed: Yes
    URI:

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