Mangan, Bozgana A. and Dunne, Margaret R. and O'Reilly, Vincent P. and Dunne, Padraic J. and Exley, Mark A. and O'Shea, Donal and Schotet, Emmanuel and Hogan, Andrew E. and Doherty, Derek G. (2013) Cutting Edge: CD1d Restriction and Th1/Th2/Th17 Cytokine Secretion by Human Vδ3 T Cells. Journal of Immunology, 191 (1). pp. 30-34. ISSN 0022-1767

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Abstract

Human γδ T cells expressing the Vδ3 TCR make up a minor lymphocyte subset in blood but are enriched in liver and in patients with some chronic viral infections and leukemias. We analyzed the frequencies, phenotypes, restriction elements, and functions of fresh and expanded peripheral blood Vδ3 T cells. Vδ3 T cells accounted for ∼0.2% of circulating T cells, included CD4+, CD8+, and CD4−CD8− subsets, and variably expressed CD56, CD161, HLA-DR, and NKG2D but neither NKG2A nor NKG2C. Vδ3 T cells were sorted and expanded by mitogen stimulation in the presence of IL-2. Expanded Vδ3 T cells recognized CD1d but not CD1a, CD1b, or CD1c. Upon activation, they killed CD1d+ target cells, released Th1, Th2, and Th17 cytokines, and induced maturation of dendritic cells into APCs. Thus, Vδ3 T cells are glycolipid-reactive T cells with distinct Ag specificities but functional similarities to NKT cells.

Item Type:	Article
Keywords:	Cutting Edge; CD1d Restriction; Th1/Th2/Th17 Cytokine Secretion; Human Vδ3 T Cells;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	12429
Identification Number:	https://doi.org/10.4049/jimmunol.1300121
Depositing User:	Andrew Hogan
Date Deposited:	17 Feb 2020 15:29
Journal or Publication Title:	Journal of Immunology
Publisher:	American Association of Immunologists
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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