Capocelli, Kelley E. and Fernando, Shahan D. and Menard-Katcher, Calies and Furuta, Glenn T. and Masterson, Joanne C. and Wartchow, Eric P.
(2015)
Ultrastructural features of eosinophilic oesophagitis: impact of
treatment on desmosomes.
Journal of Clinical Pathology, 68 (1).
ISSN 1472-4146
Abstract
Aims—A growing body of evidence suggests a role for altered epithelial barrier function in the
pathophysiology of eosinophilic oesophagitis (EoE), but few have described the epithelial
structure during inflammation. The purpose of this study was to define ultrastructural features of
active, inactive EoE and control subject’s oesophageal epithelia.
Methods—We prospectively enrolled patients undergoing diagnostic upper endoscopy for
evaluation of EoE. Mucosal pinch biopsies were obtained from the distal oesophagus and
processed for routine histology and electron microscopic assessment. Clinical features of enrolled
subjects were analysed and subjects were divided into four groups: normal, gastroesophageal
reflux disease (GERD), inactive EoE and active EoE. Representative photomicrographs of the
basal and superficial epithelia were reviewed for abnormalities. Desmosomes were quantified on
the surface of epithelia three to four prickle-cell layers above the basal layer. Results—Twenty-nine paediatric cases (ages 2–18 years) were enrolled in the study. We
observed a significant decrease in the number of desmosomes per cell (DPC) of subjects with
active EoE compared with inactive EoE, GERD and normal epithelia. With respect to DPC, no
significant differences were found between inactive EoE compared with GERD or normal
subjects. Additional ultrastructural features observed included epithelial microplicae and evidence
of eosinophil transmigration, degranulation, and sombrero formation.
Conclusions—Consistent with clinical and molecular findings, our ultrastructural data provide
support for an altered oesophageal barrier in paediatric cases with active EoE, which may improve
following treatment
| Item Type: |
Article
|
| Additional Information: |
This is the preprint version of the published article. |
| Keywords: |
Ultrastructural features; eosinophilic oesophagitis; impact;
treatment; desmosomes; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
12478 |
| Identification Number: |
https://doi.org/10.1136/jclinpath-2014-202586 |
| Depositing User: |
Joanne Masterson
|
| Date Deposited: |
26 Feb 2020 15:57 |
| Journal or Publication Title: |
Journal of Clinical Pathology |
| Publisher: |
BMJ Publishing Group |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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