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    Ultrastructural features of eosinophilic oesophagitis: impact of treatment on desmosomes

    Capocelli, Kelley E. and Fernando, Shahan D. and Menard-Katcher, Calies and Furuta, Glenn T. and Masterson, Joanne C. and Wartchow, Eric P. (2015) Ultrastructural features of eosinophilic oesophagitis: impact of treatment on desmosomes. Journal of Clinical Pathology, 68 (1). ISSN 1472-4146

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    Aims—A growing body of evidence suggests a role for altered epithelial barrier function in the pathophysiology of eosinophilic oesophagitis (EoE), but few have described the epithelial structure during inflammation. The purpose of this study was to define ultrastructural features of active, inactive EoE and control subject’s oesophageal epithelia. Methods—We prospectively enrolled patients undergoing diagnostic upper endoscopy for evaluation of EoE. Mucosal pinch biopsies were obtained from the distal oesophagus and processed for routine histology and electron microscopic assessment. Clinical features of enrolled subjects were analysed and subjects were divided into four groups: normal, gastroesophageal reflux disease (GERD), inactive EoE and active EoE. Representative photomicrographs of the basal and superficial epithelia were reviewed for abnormalities. Desmosomes were quantified on the surface of epithelia three to four prickle-cell layers above the basal layer. Results—Twenty-nine paediatric cases (ages 2–18 years) were enrolled in the study. We observed a significant decrease in the number of desmosomes per cell (DPC) of subjects with active EoE compared with inactive EoE, GERD and normal epithelia. With respect to DPC, no significant differences were found between inactive EoE compared with GERD or normal subjects. Additional ultrastructural features observed included epithelial microplicae and evidence of eosinophil transmigration, degranulation, and sombrero formation. Conclusions—Consistent with clinical and molecular findings, our ultrastructural data provide support for an altered oesophageal barrier in paediatric cases with active EoE, which may improve following treatment

    Item Type: Article
    Additional Information: This is the preprint version of the published article.
    Keywords: Ultrastructural features; eosinophilic oesophagitis; impact; treatment; desmosomes;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 12478
    Identification Number:
    Depositing User: Joanne Masterson
    Date Deposited: 26 Feb 2020 15:57
    Journal or Publication Title: Journal of Clinical Pathology
    Publisher: BMJ Publishing Group
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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