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    A simple umbelliferone based fluorescent probe for the detection of nitroreductase


    Sedgwick, Adam C. and Hayden, Alex and Hill, Barry and Bull, Steven D. and Elmes, Robert B.P. and James, Tony D. (2018) A simple umbelliferone based fluorescent probe for the detection of nitroreductase. Frontiers of Chemical Science and Engineering, 12. pp. 311-314. ISSN 2095-0187

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    Abstract

    A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

    Item Type: Article
    Keywords: Off-On Probe; Hytixic Tumor; Cancer Therapy; Living Cells; Peroxynitrite;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 12530
    Identification Number: https://doi.org/10.1007/s11705-017-1697-0
    Depositing User: Robert Elmes
    Date Deposited: 06 Mar 2020 16:52
    Journal or Publication Title: Frontiers of Chemical Science and Engineering
    Publisher: Springer
    Refereed: Yes
    URI:

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