Abukrees, Fathi and Kozlowski, Gregor and Meade, Conor
(2018)
Characterization of diverse ploidy in the arctic-alpine
Arenaria ciliata species complex (Caryophyllaceae) using
shoot meristem staining and flow cytometry analysis of
archived frozen tissue.
Plant Species Biology, 33.
pp. 144-152.
ISSN 0913-557X
Abstract
Ploidy levels were analyzed in 21 European populations of the Arenaria ciliata complex
using baseline chromosome counts derived from Feulgen staining of HCl-treated shoot
meristems and calibrated flow-cytometry analysis of fresh and archival frozen tissue.
Calibration with two to three control samples of different ploidy facilitated rapid identification of ploidy states in unknown samples. Observed ploidy levels varied from
2N = 40–200, with the majority of populations showing 2N = 40–80. High-altitude populations collectively showed the full range of ploidy states, but at low elevations only
lower ploidy levels were observed. Populations with the highest observed ploidy contained the greatest observed phylogenetic diversity in the western and eastern Alps.
Multiple polyploidization events are inferred in the continental European metapopulation, with lower, more stable ploidy characteristic of the west and north. The method
deployed provides an effective approach to ploidy analysis for archival desiccated/frozen tissue samples from biogeographic collections.
| Item Type: |
Article
|
| Keywords: |
chloroplast; Holocene; phylogeography; polyploidy; postglacial; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
12603 |
| Identification Number: |
https://doi.org/10.1111/1442-1984.12200 |
| Depositing User: |
Dr. Conor Meade
|
| Date Deposited: |
24 Mar 2020 12:48 |
| Journal or Publication Title: |
Plant Species Biology |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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