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    RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification

    White, Martin A and Azeroglu, Benura and Lopez-Vernaza, Manuel A. and Hasan, A.M. Mahedi and Leach, David R.F, (2018) RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplification. Nucleic Acids Research, 46 (13). pp. 6670-6682. ISSN 0305-1048

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    DNA double-strand break (DSB) repair is critical for cell survival. A diverse range of organisms from bacteria to humans rely on homologous recombination for accurate DSB repair. This requires both coordinate action of the two ends of a DSB and stringent control of the resultant DNA replication to prevent unwarranted DNA amplification and aneuploidy. In Escherichia coli, RecBCD enzyme is responsible for the initial steps of homologous recombination. Previous work has revealed recD mutants to be nuclease defective but recombination proficient. Despite this proficiency, we show here that a recD null mutant is defective for the repair of a two-ended DSB and that this defect is associated with unregulated chromosome amplification and defective chromosome segregation. Our results demonstrate that RecBCD plays an important role in avoiding this amplification by coordinating the two recombining ends in a manner that prevents divergent replication forks progressing away from the DSB site.

    Item Type: Article
    Keywords: RecBCD coordinates; repair; two ends; DNA; double-strand break; preventing; aberrant; chromosome amplification;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13224
    Identification Number:
    Depositing User: IR Editor
    Date Deposited: 14 Sep 2020 14:04
    Journal or Publication Title: Nucleic Acids Research
    Publisher: Oxford University Press
    Refereed: Yes

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