Liu, Dongyue and Byrne, Hugh J and O'Neill, Luke and Hennelly, Bryan M. (2018) Investigation of wavenumber calibration for Raman spectroscopy using a polymer reference. Proceedings of SPIE. p. 1068027. ISSN 0277-786X

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Abstract

Raman spectroscopy is an optical technique that can be used to evaluate the biomolecular composition of tissue and cell samples in a real-time and non-invasive manner. Subtle differences between datasets of spectra obtained from related cell groups can be identified using multivariate statistical algorithms. Such techniques are highly sensitive to small errors, however, and, therefore, the classification sensitivity of Raman spectroscopy can be significantly impacted by miscalibration of the optical system due to small misalignments of the optical elements and/or variation in ambient temperature. Wavenumber calibration is often achieved by recording the spectrum from a wavenumber reference standard, such as 4-acetamidophenol or benzene, which contains numerous sharp peaks in the fingerprint region. Here, we investigate a commercial polymer slide as a wavenumber reference standard for the calibration of Raman spectra. The Raman spectrum of this slide contains numerous sharp peaks in the fingerprint region. Unlike many other reference standards, the polymer slide is non-hazardous, has an indefinite lifetime, and is designed in the shape of a glass slide used for microscopy. We evaluate this reference in terms of accuracy and repeatability, and we compare with the established 4-Acetamidophenol wavenumber reference.

Item Type:	Article
Additional Information:	This is the preprint version of the published article, which is available at: Dongyue Liu, Hugh J. Byrne, Luke O'Neill, and Bryan Hennelly "Investigation of wavenumber calibration for Raman spectroscopy using a polymer reference", Proc. SPIE 10680, Optical Sensing and Detection V, 1068027 (9 May 2018); https://doi.org/10.1117/12.2307574 . This Conference Paper has been made available for free and open access by the Radiation and Environmental Science Centre at ARROW@TU Dublin.
Keywords:	Raman spectroscopy; wavenumber calibration; peak stability; wavenumber reference; 4-Acetamidophenol;
Academic Unit:	Faculty of Science and Engineering > Computer Science Faculty of Science and Engineering > Electronic Engineering Faculty of Science and Engineering > Research Institutes > Hamilton Institute
Item ID:	13439
Identification Number:	https://doi.org/10.1117/12.2307574
Depositing User:	Dr. Bryan Hennelly
Date Deposited:	09 Oct 2020 16:53
Journal or Publication Title:	Proceedings of SPIE
Publisher:	SPIE
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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