Xu, Linan and Nitika and Hasin, Naushaba and Cuskelly, Daragh and Wolfgeher, Donald and Doyle, Sean and Moynagh, Paul N. and Perrett, Sarah and Jones, Gary W. and Truman, Andrew W. (2019) Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress. Scientific Reports, 9 (16260). ISSN 2045-2322

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Abstract

Hsp70 is a highly conserved molecular chaperone critical for the folding of new and denatured proteins. While traditional models state that cells respond to stress by upregulating inducible HSPs, this response is relatively slow and is limited by transcriptional and translational machinery. Recent studies have identifed a number of post-translational modifcations (PTMs) on Hsp70 that act to fne-tune its function. We utilized mass spectrometry to determine whether yeast Hsp70 (Ssa1) is diferentially modifed upon heat shock. We uncovered four lysine residues on Ssa1, K86, K185, K354 and K562 that are deacetylated in response to heat shock. Mutation of these sites cause a substantial remodeling of the Hsp70 interaction network of co-chaperone partners and client proteins while preserving essential chaperone function. Acetylation/deacetylation at these residues alter expression of other heat-shock induced chaperones as well as directly infuencing Hsf1 activity. Taken together our data suggest that cells may have the ability to respond to heat stress quickly though Hsp70 deacetylation, followed by a slower, more traditional transcriptional response.

Item Type:	Article
Additional Information:	Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Cite as: Xu, L., Nitika, Hasin, N. et al. Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress. Sci Rep 9, 16260 (2019). https://doi.org/10.1038/s41598-019-52545-3
Keywords:	Rapid; deacetylation; yeast; Hsp70; mediates; cellular; heat stress;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	13449
Identification Number:	https://doi.org/10.1038/s41598-019-52545-3
Depositing User:	Professor Paul Moynagh
Date Deposited:	22 Oct 2020 15:16
Journal or Publication Title:	Scientific Reports
Publisher:	Nature Publishing Group
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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