MURAL - Maynooth University Research Archive Library



    Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae


    Sheehan, Gerard and Konings, Mickey and Lim, Wilson and Fahal, Ahmed and Kavanagh, Kevin and van de Sande, Wendy W.J. (2020) Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Neglected Tropical Diseases, 14 (4). e0008190. ISSN 1935-2727

    [img]
    Preview
    Download (2MB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection.

    Item Type: Article
    Additional Information: © 2020 Sheehan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation: Sheehan G, Konings M, Lim W, Fahal A, Kavanagh K, van de Sande WWJ (2020) Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. PLoS Negl Trop Dis 14(4): e0008190. https://doi.org/10.1371/journal.pntd.0008190
    Keywords: Proteomic analysis; Madurella mycetomatis grain formation; Galleria mellonella larvae; Mycetoma;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13673
    Identification Number: https://doi.org/10.1371/journal.pntd.0008190
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 26 Nov 2020 16:18
    Journal or Publication Title: PLoS Neglected Tropical Diseases
    Publisher: Public Library of Science
    Refereed: Yes
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year