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    Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics

    Morrin, Sinead T and Owens, Rebecca A. and Berre, Marie Le and Gerlach, Jared Q and Joshi, Lokesh and Bode, Lars and Irwin, Jane A and Hickey, Rita M. (2019) Interrogation of Milk-Driven Changes to the Proteome of Intestinal Epithelial Cells by Integrated Proteomics and Glycomics. Journal of Agricultural and Food Chemistry, 67. pp. 1902-1917. ISSN 0021-8561

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    Bovine colostrum is a rich source of bioactive components which are important in the development of the intestine, in stimulating gut structure and function and in preparing the gut surface for subsequent colonization of microbes. What is not clear, however, is how colostrum may affect the repertoire of receptors and membrane proteins of the intestinal surface and the post-translational modifications associated with them. In the present work, we aimed to characterize the surface receptor and glycan profile of human HT-29 intestinal cells after exposure to a bovine colostrum fraction (BCF) by means of proteomic and glycomic analyses. Integration of label-free quantitative proteomic analysis and lectin array profiles confirmed that BCF exposure results in changes in the levels of glycoproteins present at the cell surface and also changes to their glycosylation pattern. This study contributes to our understanding of how milk components may regulate intestinal cells and prime them for bacterial interaction.

    Item Type: Article
    Keywords: bovine colostrum; proteome; receptor; glycomics; lectin array; milk;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13883
    Identification Number:
    Depositing User: Rebecca Owens
    Date Deposited: 28 Jan 2021 15:43
    Journal or Publication Title: Journal of Agricultural and Food Chemistry
    Publisher: American Chemical Society
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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