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    Quantitative proteomic profiling of ochratoxin A repression in Penicillium nordicum by protective cultures


    Delgado, Josué and Núñez, Felix and Asensio, Miguel A. and Owens, Rebecca A. (2019) Quantitative proteomic profiling of ochratoxin A repression in Penicillium nordicum by protective cultures. International Journal of Food Microbiology, 305. ISSN 0168-1605

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    Abstract

    Dry-cured meat products are usually contaminated with moulds during ripening. Although fungal development contributes to the desired sensory characteristics, some moulds, such as Penicillium nordicum are able to produce ochratoxin A (OTA) on meat products. Therefore, strategies to prevent OTA contamination in ripened meat products are required. Microorganisms isolated from these meat products can be adequate as biocontrol agents, given that no negative sensory impact is expected. The PgAFP antifungal protein-producer Penicillium chrysogenum (Pc) and Debaryomyces hansenii (Dh) have been shown to successfully inhibit toxigenic moulds. However, scarce information about the mechanism of action of these biocontrol agents on toxigenic mould inhibition is available. Comparative proteomic analysis is a powerful tool to investigate the physiological response of microorganisms to stimuli. Proteomic analysis was carried out on P. nordicum co-cultured with Pc, Dh, PgAFP, and their combinations on a dry-cured ham-based medium. Additionally, OTA production by P. nordicum in the different cultures was measured. The individual inoculation of Pc or Dh repressed OTA production by P. nordicum by 5 and 3.15 fold, respectively. A total of 2844 unique P. nordicum proteins were identified by proteomic analysis. The impact of the biocontrol agents on the proteome of P. nordicum was higher for Pc-containing cultures, followed by Dh-containing treatments. PgAFP alone had minimal impact on the proteome of P. nordicum. Proteomic analyses indicated Pc repressed P. nordicum OTA production through nutrient competition, potentially reducing glucose availability. Data also suggest that Dh and Pc inhibited P. nordicum through cell wall integrity impairment. Both Pc and Dh seem to hamper P. nordicum secondary metabolism (SM) as indicated by lower levels of MAP kinases and SM-associated proteins found in the co-inoculated P. nordicum. This work paves the way to use antifungal agents in the most efficient way to prevent OTA formation in meat products.

    Item Type: Article
    Keywords: Debaryomyces hansenii; Penicillium chrysogenum; Ochratoxin; PgAFP; Protective cultures; Proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13884
    Identification Number: https://doi.org/10.1016/j.ijfoodmicro.2019.108243
    Depositing User: Rebecca Owens
    Date Deposited: 01 Feb 2021 12:04
    Journal or Publication Title: International Journal of Food Microbiology
    Publisher: Elsevier
    Refereed: Yes
    URI:

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