Delgado, Josué and Núñez, Felix and Asensio, Miguel A. and Owens, Rebecca A.
(2019)
Quantitative proteomic profiling of ochratoxin A repression in Penicillium nordicum by protective cultures.
International Journal of Food Microbiology, 305.
ISSN 0168-1605
Abstract
Dry-cured meat products are usually contaminated with moulds during ripening. Although fungal development
contributes to the desired sensory characteristics, some moulds, such as Penicillium nordicum are able to produce
ochratoxin A (OTA) on meat products. Therefore, strategies to prevent OTA contamination in ripened meat
products are required. Microorganisms isolated from these meat products can be adequate as biocontrol agents,
given that no negative sensory impact is expected. The PgAFP antifungal protein-producer Penicillium chrysogenum (Pc) and Debaryomyces hansenii (Dh) have been shown to successfully inhibit toxigenic moulds. However,
scarce information about the mechanism of action of these biocontrol agents on toxigenic mould inhibition is
available. Comparative proteomic analysis is a powerful tool to investigate the physiological response of microorganisms to stimuli. Proteomic analysis was carried out on P. nordicum co-cultured with Pc, Dh, PgAFP, and
their combinations on a dry-cured ham-based medium. Additionally, OTA production by P. nordicum in the
different cultures was measured. The individual inoculation of Pc or Dh repressed OTA production by P. nordicum by 5 and 3.15 fold, respectively. A total of 2844 unique P. nordicum proteins were identified by proteomic
analysis. The impact of the biocontrol agents on the proteome of P. nordicum was higher for Pc-containing
cultures, followed by Dh-containing treatments. PgAFP alone had minimal impact on the proteome of P. nordicum. Proteomic analyses indicated Pc repressed P. nordicum OTA production through nutrient competition,
potentially reducing glucose availability. Data also suggest that Dh and Pc inhibited P. nordicum through cell
wall integrity impairment. Both Pc and Dh seem to hamper P. nordicum secondary metabolism (SM) as indicated
by lower levels of MAP kinases and SM-associated proteins found in the co-inoculated P. nordicum. This work
paves the way to use antifungal agents in the most efficient way to prevent OTA formation in meat products.
| Item Type: |
Article
|
| Keywords: |
Debaryomyces hansenii;
Penicillium chrysogenum;
Ochratoxin;
PgAFP;
Protective cultures;
Proteomics; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
13884 |
| Identification Number: |
https://doi.org/10.1016/j.ijfoodmicro.2019.108243 |
| Depositing User: |
Rebecca Owens
|
| Date Deposited: |
01 Feb 2021 12:04 |
| Journal or Publication Title: |
International Journal of Food Microbiology |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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