MURAL - Maynooth University Research Archive Library

    A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin

    Dix, Philip and Joo, Ferenc and Maliga, Pal (1977) A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Molecular and General Genetics MGG, 157. pp. 285-290. ISSN 0026-8925

    Download (527kB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    Cell lines resistant to 50 gg ml- 1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis. Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells. KR103 is defective in morphogenesis and plastid development.

    Item Type: Article
    Keywords: cell line; Nicotiana sylvestris; resistance; kanamycin; streptomycin;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 13908
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 01 Feb 2021 14:10
    Journal or Publication Title: Molecular and General Genetics MGG
    Publisher: Springer Verlag
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads