Harns, Emily and Roberts, Joseph and Bermingham, Rachel and Hogan, Andrew E. and O'Shea, Donal and O'Neill, Luke and Fallon, Padraic G.
(2020)
Role for Retinoic Acid-Related Orphan Receptor Alpha (RORα) Expressing Macrophages in Diet-Induced Obesity.
Frontiers in Immunology, 11 (1966).
pp. 1-13.
ISSN 1664-3224
Abstract
The transcription factor RORα plays an important role in regulating circadian rhythm,
inflammation, metabolism, and cellular development. Herein we show a role for
RORα-expressing macrophages in the adipose tissue in altering the metabolic state
of mice on a high-fat diet. The expression of Rora and RORA is elevated in white
adipose tissue from obese mice and humans when compared to lean counterparts.
When fed a high-fat diet Rora reporter mice revealed increased expression of Rora-YFP
in macrophages in white adipose tissue deposits. To further define the potential role
for Rora-expressing macrophages in the generation of an aberrant metabolic state
Rorafl/flLysMCre/+ mice, which do not express Rora in myeloid cells, were maintained
on a high-fat diet, and metabolic parameters assessed. These mice had significantly
impaired weight gain and improved metabolic parameters in comparison to Rorafl/fl
control mice. Further analysis of the immune cell populations within white adipose tissue
deposits demonstrates a decrease in inflammatory adipose tissue macrophages (ATM).
In obese reporter mouse there was increased in Rora-YFP expressing ATM in adipose
tissue. Analysis of peritoneal macrophage populations demonstrates that within the
peritoneal cavity Rora-expression is limited to myeloid-derived macrophages, suggesting
a novel role for RORα in macrophage development and activation, which can impact on
metabolism, and inflammation.
| Item Type: |
Article
|
| Keywords: |
RORα; macrophage; inflammation; metabolism; obesity; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
14910 |
| Identification Number: |
https://doi.org/10.3389/fimmu.2020.01966 |
| Depositing User: |
Andrew Hogan
|
| Date Deposited: |
11 Oct 2021 11:55 |
| Journal or Publication Title: |
Frontiers in Immunology |
| Publisher: |
Frontiers Media |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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