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    Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy


    Dowling, Paul and Zweyer, Margit and Raucamp, Maren and Henry, Michael and Meleady, Paula and Swandulla, Dieter and Ohlendieck, Kay (2020) Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy. European Journal of Cell Biology, 99 (151059). ISSN 0171-9335

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    Abstract

    The X-linked inherited muscle wasting disease Duchenne muscular dystrophy, which is caused by primary abnormalities in the membrane cytoskeletal protein dystrophin, is a multi-system disorder. Highly progressive forms of dystrophinopathy are associated with a complex secondary pathophysiology, including renal dysfunction. It was therefore of interest to carry out a systematic survey of potential proteome-wide changes in the kidney of the established mdx-4cv mouse model of dystrophinopathy. Of 5878 mass spectrometrically identified kidney proteins, 82 versus 142 proteins were shown to be decreased or increased, respectively, in association with muscular dystrophy. The most decreased versus increased protein species are the ACSM3 isoform of mitochondrial acyl-coenzyme A synthetase and the FABP1 isoform of fatty acid binding protein, respectively. Both proteomic findings were verified by immunofluorescence microscopy and immunoblot analysis. Interestingly, haematoxylin/eosin staining indicated diffuse whitish deposits in the mdx-4cv kidney, and an increased intensity of Sudan Black labelling of kidney cells revealed ectopic fat deposition. Although the proteomic results and cell biological findings do not demonstrate a direct functional link between increased FABP1 and fat accumulation, the results suggest that the up-regulation of FABP1 may be related to abnormal fat metabolism. This makes FABP1 potentially a novel pathobiochemical indicator for studying kidney abnormalities in the mdx-4cv model of dystrophinopathy.

    Item Type: Article
    Keywords: Dystrophin; Dystrophinopathy; Ectopic fat; FABP1; Fatty acid binding protein; Kidney disease;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 14962
    Identification Number: https://doi.org/10.1016/j.ejcb.2019.151059
    Depositing User: Paul Dowling
    Date Deposited: 26 Oct 2021 16:21
    Journal or Publication Title: European Journal of Cell Biology
    Publisher: Urban & Fischer
    Refereed: Yes
    URI:

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