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    Proteome-wide Changes in the mdx-4cv Spleen due to Pathophysiological Cross Talk with Dystrophin-Deficient Skeletal Muscle


    Dowling, Paul and Gargan, Stephen and Zweyer, Margit and Henry, Michael and Meleady, Paula and Swandulla, Dieter and Ohlendieck, Kay (2020) Proteome-wide Changes in the mdx-4cv Spleen due to Pathophysiological Cross Talk with Dystrophin-Deficient Skeletal Muscle. iScience, 23 (101500). ISSN 2589-0042

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    Abstract

    Duchenne muscular dystrophy is primarily characterized by progressive muscle wasting due to deficiency in the membrane cytoskeletal protein dystrophin but is also associated with body-wide cellular disturbances in a variety of non-muscle tissues. In this study, we have focused on the comparative proteomic analysis of the spleen and established considerable changes in this crucial secondary lymphoid organ from the genetic mdx-4cv mouse model of dystrophinopathy. An apparent short isoform of dystrophin and associated glycoproteins were identified in spleen by mass spectrometry but appear not be affected in muscular dystrophy. In contrast, the mdx-4cv spleen showed significant proteome-wide changes in other protein species that are involved in metabolism, signaling, and cellular architecture. Since the spleen plays a key role in the immune response, these proteomic alterations may reflect pathophysiological cross talk between the lymphoid system and dystrophic muscles, which are affected by both fiber degeneration and inflammation.

    Item Type: Article
    Keywords: Proteome; Changes; mdx-4cv Spleen; Pathophysiological; Cross Talk; Dystrophin-Deficient; Skeletal Muscle;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 14963
    Identification Number: https://doi.org/10.1016/j.isci. 2020.101500
    Depositing User: Paul Dowling
    Date Deposited: 26 Oct 2021 16:32
    Journal or Publication Title: iScience
    Publisher: Cell Press
    Refereed: Yes
    URI:

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