Dowling, Paul and Gargan, Stephen and Zweyer, Margit and Henry, Michael and Meleady, Paula and Swandulla, Dieter and Ohlendieck, Kay
(2020)
Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen.
STAR Protocols, 1 (100196).
ISSN 2666-1667
Abstract
This protocol describes the comparative proteomic profiling of the spleen of wild
type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample
preparation for bottom-up proteomic mass spectrometry experiments, including
homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass
spectrometric analysis and bioinformatic evaluation.
For complete details on the use and execution of this protocol, please refer to
Dowling et al. (2020).
| Item Type: |
Article
|
| Keywords: |
Protocol; Bottom-Up; Proteomic;
Analysis; Mouse Spleen; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
14964 |
| Identification Number: |
https://doi.org/10.1016/ j.xpro.2020.100196 |
| Depositing User: |
Paul Dowling
|
| Date Deposited: |
26 Oct 2021 16:39 |
| Journal or Publication Title: |
STAR Protocols |
| Publisher: |
Cell Press |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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