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    Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen


    Dowling, Paul and Gargan, Stephen and Zweyer, Margit and Henry, Michael and Meleady, Paula and Swandulla, Dieter and Ohlendieck, Kay (2020) Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen. STAR Protocols, 1 (100196). ISSN 2666-1667

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    Abstract

    This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020).

    Item Type: Article
    Keywords: Protocol; Bottom-Up; Proteomic; Analysis; Mouse Spleen;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 14964
    Identification Number: https://doi.org/10.1016/ j.xpro.2020.100196
    Depositing User: Paul Dowling
    Date Deposited: 26 Oct 2021 16:39
    Journal or Publication Title: STAR Protocols
    Publisher: Cell Press
    Refereed: Yes
    URI:

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