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    Exposure to n-chlorotaurine induces oxidative stress responses in aspergillus fumigatus

    Sheehan, Gerard and Nagl, Markus and Kavanagh, Kevin (2019) Exposure to n-chlorotaurine induces oxidative stress responses in aspergillus fumigatus. Journal of Medical Microbiology, 68. pp. 279-288. ISSN 0022-2615

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    Purpose. The neutrophil-derived oxidant N-chlorotaurine (NCT) displays remarkable in vivo tolerability and efficacy against a range of pathogens. The aim of this study was to characterize the response of the pulmonary pathogen Aspergillus fumigatus to NCT. Methodology. The effect of NCT on the growth and viability of A. fumigatus was characterized. NCT-induced alteration of amino acids and gliotoxin from A. fumigatus mycelium was assessed. Label-free shotgun quantitative proteomic analysis was performed on A. fumigatus exposed to NCT for 24 h. Results. Incubation of A. fumigatus with NCT at concentrations ranging from 6.8 to 55 mM decreased conidial growth and viability, and mycelium biomass relative to the controls. Exposure to NCT (13.77 mM) resulted in increased amino acids and gliotoxin levels from A. fumigatus mycelium. Exposure of A. fumigatus mycelium to NCT (6.8 mM) revealed an enrichment in proteins associated with the ribosome, transcription and translation and non-ribosomal peptide biosynthesis (e.g. Pes1, Pes3), which play an essential role in oxidative stress resistance in A. fumigatus. A decrease in the abundance of proteins associated with fumagillin and pseurotin biosynthesis highlighted the anti-virulence activity of NCT. Conclusion. These results indicate that NCT induces an oxidative stress response in A. fumigatus as evidenced by alterations in the proteome and inhibits conidial and mycelial growth. Clinical investigations of topical application of NCT to treat Aspergillus infections are encouraged.

    Item Type: Article
    Keywords: Exposure; N-chlorotaurine; oxidative stress; Aspergillus fumigatus;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 14990
    Identification Number:
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 08 Nov 2021 12:38
    Journal or Publication Title: Journal of Medical Microbiology
    Publisher: Society for General Microbiology
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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