Hanson, Sara J and Cinnéide, Eoin Ó and Salzberg, Letal I and Wolfe, Kenneth H and McGowan, Jamie and Fitzpatrick, David A. and Matlin, Kate and Berman, J (2021) Genomic diversity, chromosomal rearrangements, and interspecies hybridization in the Ogataea polymorpha species complex. G3 Genes|Genomes|Genetics, 11 (8). pp. 1-18. ISSN 2160-1836

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Abstract

The methylotrophic yeast Ogataea polymorpha has long been a useful system for recombinant protein production, as well as a model system for methanol metabolism, peroxisome biogenesis, thermotolerance, and nitrate assimilation. It has more recently become an important model for the evolution of mating-type switching. Here, we present a population genomics analysis of 47 isolates within the O. polymorpha species complex, including representatives of the species O. polymorpha, Ogataea parapolymorpha, Ogataea haglerorum, and Ogataea angusta. We found low levels of nucleotide sequence diversity within the O. polymorpha species complex and identified chromosomal rearrangements both within and between species. In addition, we found that one isolate is an interspecies hybrid between O. polymorpha and O. parapolymorpha and present evidence for loss of heterozygosity following hybridization.

Item Type:	Article
Keywords:	Ogataea; population genomics; mating-type switching; interspecies hybridization; chromosomal rearrangements;
Academic Unit:	Faculty of Science and Engineering > Biology Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	16182
Identification Number:	https://doi.org/10.1093/g3journal/jkab211
Depositing User:	David Fitzpatrick
Date Deposited:	27 Jun 2022 11:53
Journal or Publication Title:	G3 Genes|Genomes|Genetics
Publisher:	Oxford University Press
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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