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    Genome sequence of the English grain aphid, Sitobion avenae and its endosymbiont Buchnera aphidicola

    Byrne, Stephen and Schughart, Maximilian and Carolan, James C. and Gaffney, Michael and Thorpe, Peter and Malloch, Gaynor and Wilkinson, Tom and McNamara, Louise and Scofield, S (2021) Genome sequence of the English grain aphid, Sitobion avenae and its endosymbiont Buchnera aphidicola. G3 Genes|Genomes|Genetics, 12 (3). pp. 1-8. ISSN 2160-1836

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    The English grain aphid, Sitobion avenae, is a major agricultural pest of wheat, barley and oats, and one of the principal vectors of barley yellow dwarf virus leading to significant reductions in grain yield, annually. Emerging resistance to and increasing regulation of insecticides has resulted in limited options for their control. Using PacBio HiFi data, we have produced a high-quality draft assembly of the S. avenae genome; generating a primary assembly with a total assembly size of 475.7 Mb, and an alternate assembly with a total assembly size of 430.8 Mb. Our primary assembly was highly contiguous with only 326 contigs and a contig N50 of 15.95 Mb. Assembly completeness was estimated at 97.7% using BUSCO analysis and 31,007 and 29,037 protein-coding genes were predicted from the primary and alternate assemblies, respectively. This assembly, which is to our knowledge the first for an insecticide resistant clonal lineage of English grain aphid, will provide novel insight into the molecular and mechanistic determinants of resistance and will facilitate future research into mechanisms of viral transmission and aphid behavior.

    Item Type: Article
    Keywords: aphid; PacBio HiFi; Sitobion avenae; genome;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 16221
    Identification Number:
    Depositing User: James Carolan
    Date Deposited: 04 Jul 2022 16:13
    Journal or Publication Title: G3 Genes|Genomes|Genetics
    Publisher: Genetics Society of America
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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