MURAL - Maynooth University Research Archive Library

    α-glutathione s-transferase (α-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat

    Clarke, H. and Egan, DA and Heffernan, M. and Doyle, Sean and Byrne, C. and Kilty, C. and Ryan, MP (1997) α-glutathione s-transferase (α-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat. Human & Experimental Toxicology, 16 (3). pp. 154-157. ISSN 0960-3271

    Download (360kB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    1. The use of the cytoplasmic enzyme, alpha glutathione s-transferase (alpha-GST) as an early index of carbon tetrachloride (CCl4) toxicity in the rat was investigated and compared with a standard enzyme, marker, aspartate aminotransferase (AST). The hepatotoxic effects of CCl4 in the rat were determined in a time and dose-response study. 2. Following CCl4 exposure, alpha-GST release was shown to be an earlier and more sensitive biomarker of hepatotoxicity than AST. 3. Significant increases in alpha-GST were detected 2 h after CCl4 exposure. Using the enzyme marker AST, this early hepatotoxic injury went undetected. At 6 and 16 h, alpha-GST was also a more sensitive indicator of hepatotoxicity than AST. 4. alpha-GST release was significantly increased at a dose of 5 microliters/kg, the lowest concentration of CCl4 administered and clearly responded in a dose-dependent manner with increasing doses of CCl4. In contrast, release of AST did not reach statistical significance until a dose of 25 microliters/kg. 5. Thus, these findings indicate that alpha-GST is a more sensitive and more accurate reflector of CCl4 induced hepatotoxicity than AST.

    Item Type: Article
    Keywords: α-glutathione; s-transferase; α-GST; asparate amino transferase; AST; hepatotoxicity; carbon tetrachloride; biomarker;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 16228
    Identification Number:
    Depositing User: Dr. Sean Doyle
    Date Deposited: 05 Jul 2022 13:49
    Journal or Publication Title: Human & Experimental Toxicology
    Publisher: SAGE Publications
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads