Clarke, H. and Egan, DA and Heffernan, M. and Doyle, Sean and Byrne, C. and Kilty, C. and Ryan, MP (1997) α-glutathione s-transferase (α-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat. Human & Experimental Toxicology, 16 (3). pp. 154-157. ISSN 0960-3271

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Abstract

1. The use of the cytoplasmic enzyme, alpha glutathione s-transferase (alpha-GST) as an early index of carbon tetrachloride (CCl4) toxicity in the rat was investigated and compared with a standard enzyme, marker, aspartate aminotransferase (AST). The hepatotoxic effects of CCl4 in the rat were determined in a time and dose-response study. 2. Following CCl4 exposure, alpha-GST release was shown to be an earlier and more sensitive biomarker of hepatotoxicity than AST. 3. Significant increases in alpha-GST were detected 2 h after CCl4 exposure. Using the enzyme marker AST, this early hepatotoxic injury went undetected. At 6 and 16 h, alpha-GST was also a more sensitive indicator of hepatotoxicity than AST. 4. alpha-GST release was significantly increased at a dose of 5 microliters/kg, the lowest concentration of CCl4 administered and clearly responded in a dose-dependent manner with increasing doses of CCl4. In contrast, release of AST did not reach statistical significance until a dose of 25 microliters/kg. 5. Thus, these findings indicate that alpha-GST is a more sensitive and more accurate reflector of CCl4 induced hepatotoxicity than AST.

Item Type:	Article
Keywords:	α-glutathione; s-transferase; α-GST; asparate amino transferase; AST; hepatotoxicity; carbon tetrachloride; biomarker;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	16228
Identification Number:	https://doi.org/10.1177/096032719701600304
Depositing User:	Dr. Sean Doyle
Date Deposited:	05 Jul 2022 13:49
Journal or Publication Title:	Human & Experimental Toxicology
Publisher:	SAGE Publications
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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