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    Baculovirus expression of parvovirus B19 (B19V) NS1: utility in confirming recent infection

    Mahon, Bernard P. and Doyle, Sean and Kavanagh, Kevin and Corcoran, Amanda and Ennis, O. (2001) Baculovirus expression of parvovirus B19 (B19V) NS1: utility in confirming recent infection. Journal of Clinical Virology, 22 (1). pp. 55-60. ISSN 1386-6532

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    Background :The presence of anti-parvovirus B19 (B19V) IgM against viral capsid proteins (VP1 and VP2) has long been used to detect recent infection. The utility of antibodies directed against B19V NS1 protein has received less attention as a serological indicator of recent infection, although anti-B19V NS1 IgG has been associated with persistent infection. Objecties : To elucidate the role of anti-B19V NS1 antibody detection in recent infection, full-length B19V NS1 was expressed and purified. The resultant antigen was used to develop both Western blot assays and microplate ELISA for the detection of NS1 antibodies. Study design : Serum specimens were obtained from individuals recently infected with B19V (children (n=16), adults (n=40)) and from 17 individuals with no evidence of recent B19V infection. All specimens were screened for anti-B19V NS1 IgG and IgM. Results : It was observed that 68.8% (11/16) of children recently infected with B19V were anti-B19V NS1 IgG seropositive. Furthermore, 27.5% (11/40) anti-B19V VP2 IgM positive specimens also contained anti-B19V NS1 IgM when tested by ELISA, while no reactivity was observed following Western blot analysis, possibly due to the absence of conformational epitopes. Conclusions : Anti-B19V NS1 IgM detection may have utility in the confirmation of recent infection with B19V.

    Item Type: Article
    Keywords: Baculovirus; ELISA; Epitopes; Immunocompromised; Pregnancy;
    Academic Unit: Faculty of Science and Engineering > Biology
    Faculty of Science and Engineering > Research Institutes > Institute of Immunology
    Item ID: 167
    Identification Number:
    Depositing User: Bernard Mahon
    Date Deposited: 04 Jan 2005
    Journal or Publication Title: Journal of Clinical Virology
    Publisher: Elsevier Science B.V.
    Refereed: Yes
    Funders: Enterprise Ireland (EI), Health Research Board (HRB)
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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